Journal: Skin Research and Technology

Article Title: Tanshinone IIA, a component of the self‐made Xiao‐Yin decoction, ameliorates psoriasis by inhibiting IL‐17/IL‐23 and PTGS2/NF‐κB/AP‐1 pathways

doi: 10.1111/srt.13577

Figure Lengend Snippet: Tan‐IIA improved psoriasis and downregulated PTGS2/NF‐κB/AP‐1 signaling. (A) Drug toxicity of tan‐IIA for 24 h was detected using CCK8 assay. (B) The impact of tan‐IIA (10 µg/mL) on the proliferation of HaCaT keratinocytes using CCK8 assay. (C) The mRNA levels of inflammatory cytokines, including IL‐17, IL‐23, TNF‐α, and IL‐6 in HaCaT keratinocytes. (D) The expression levels of PTGS2, AP‐1, p‐AP‐1, p65, and p‐p65 in HaCaT keratinocytes. Data are expressed as mean ± SD ( n = 3). # P < 0.05 versus Control group, * P < 0.05 versus M‐5 group.

Article Snippet: When the cells reached 80%–90% confluence, they were divided into four groups: (1) Control group: Cells were cultured under standard conditions. (2) M‐5 group: To create a psoriasis‐like keratinocytes model, HaCaT keratinocytes were exposed to a combination of cytokines (IL‐17A, IL‐22, oncostatin M, IL‐1α, and TNF‐α) (PeproTech, USA) at a concentration of 2.5 ng/mL for 12 h. (3) Tan‐IIA group: The cells were treated with 10 μg/mL of tan‐IIA (HY‐N0135, MedChem Express, USA) for 24 h, followed by a 12‐h M‐5 treatment. (4) MTX group: The cells were treated with 2 μM MTX for 24 h, followed by a 12‐h M‐5 treatment.

Techniques: CCK-8 Assay, Expressing, Control

Journal: Skin Research and Technology

Article Title: Tanshinone IIA, a component of the self‐made Xiao‐Yin decoction, ameliorates psoriasis by inhibiting IL‐17/IL‐23 and PTGS2/NF‐κB/AP‐1 pathways

doi: 10.1111/srt.13577

Figure Lengend Snippet: Tan‐IIA improved psoriasis through PTGS2. (A–B) The expression level of PTGS2 was evaluated using western blotting. (C) The impact of tan‐IIA on the proliferation of HaCaT keratinocytes using CCK8 assay. (D) The mRNA levels of inflammatory cytokines, including IL‐23, TNF‐α, and IL‐6 in HaCaT keratinocytes. (E) The expression levels of AP‐1, p‐AP‐1, p65, and p‐p65 in HaCaT keratinocytes. Data are expressed as mean ± SD ( n = 3). # P < 0.05 versus M‐5 group, * P < 0.05 versus M‐5+Tan‐IIA+oe‐NC group.

Article Snippet: When the cells reached 80%–90% confluence, they were divided into four groups: (1) Control group: Cells were cultured under standard conditions. (2) M‐5 group: To create a psoriasis‐like keratinocytes model, HaCaT keratinocytes were exposed to a combination of cytokines (IL‐17A, IL‐22, oncostatin M, IL‐1α, and TNF‐α) (PeproTech, USA) at a concentration of 2.5 ng/mL for 12 h. (3) Tan‐IIA group: The cells were treated with 10 μg/mL of tan‐IIA (HY‐N0135, MedChem Express, USA) for 24 h, followed by a 12‐h M‐5 treatment. (4) MTX group: The cells were treated with 2 μM MTX for 24 h, followed by a 12‐h M‐5 treatment.

Techniques: Expressing, Western Blot, CCK-8 Assay

Journal: Skin Research and Technology

Article Title: Tanshinone IIA, a component of the self‐made Xiao‐Yin decoction, ameliorates psoriasis by inhibiting IL‐17/IL‐23 and PTGS2/NF‐κB/AP‐1 pathways

doi: 10.1111/srt.13577

Figure Lengend Snippet: Tan‐IIA attenuated IMQ‐induced inflammation, IL‐17/IL‐23, and PTGS2/NF‐κB/AP‐1 signaling in mice skin. (A) The expression levels of IL‐17 and IL‐23 in the skin tissues were evaluated. (B) The protein expression levels of PTGS2, AP‐1, p‐AP‐1, p65, and p‐p65 were assessed in the skin tissues. (C) The levels of inflammatory cytokines in mouse serum were performed using ELISA. Data are expressed as mean ± SD ( n = 5). # P < 0.05 versus Sham group, * P < 0.05 versus Model group, ^ P < 0.05 versus MTX group.

Article Snippet: When the cells reached 80%–90% confluence, they were divided into four groups: (1) Control group: Cells were cultured under standard conditions. (2) M‐5 group: To create a psoriasis‐like keratinocytes model, HaCaT keratinocytes were exposed to a combination of cytokines (IL‐17A, IL‐22, oncostatin M, IL‐1α, and TNF‐α) (PeproTech, USA) at a concentration of 2.5 ng/mL for 12 h. (3) Tan‐IIA group: The cells were treated with 10 μg/mL of tan‐IIA (HY‐N0135, MedChem Express, USA) for 24 h, followed by a 12‐h M‐5 treatment. (4) MTX group: The cells were treated with 2 μM MTX for 24 h, followed by a 12‐h M‐5 treatment.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay